Serveur d'exploration H2N2

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction

Identifieur interne : 001F32 ( Main/Exploration ); précédent : 001F31; suivant : 001F33

Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction

Auteurs : E. C. J. Claas ; M. J. W. Sprenger ; G. E. M. Kletera ; R. Van Beek ; W. G. V. Quint ; N. Masurela

Source :

RBID : ISTEX:2333ADD4E632599E5B80D99083E693ED19C1EF4D

Descripteurs français

English descriptors

Abstract

Abstract: The aim of this study was to develop a polymerase chain reaction for specific detection of influenza A, B, and C RNA genomes. Three primer sets were selected from conserved regions of the genome coding for the non-structural proteins and were tested on 61 influenza A (22 H1N1, 9 H2N2, and 30 H3N2), 11 influenza B, and three influenza C isolates. Specific amplified products were obtained with all these strains after electrophoresis on a 2% agarose gel. The specificity of the reaction was increased by hybridization with oligonucleotide probes. When nucleic acids from a variety of micro-organisms from the respiratory tract were subjected to the PCR with these primers, no specific amplified products were generated. The sensitivity of the technique was found to be at the subpicogram level. The RNA-PCR was applied to 21 clinical specimens from patients with a culture/IF proven influenza infection. Six influenza A positive patients and 13 influenza B positive patients could be confirmed in the RNA-PCR. In two cases, influenza B positive IF specimens were found negative by the PCR. No virus could be isolated on eggs or tissue culture from these samples. RNA-PCR is a specific and sensitive technique for the detection of influenza virus genomes.

Url:
DOI: 10.1016/0166-0934(92)90120-3


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

<record>
<TEI wicri:istexFullTextTei="biblStruct">
<teiHeader>
<fileDesc>
<titleStmt>
<title>Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction</title>
<author>
<name sortKey="Claas, E C J" sort="Claas, E C J" uniqKey="Claas E" first="E. C. J." last="Claas">E. C. J. Claas</name>
</author>
<author>
<name sortKey="Sprenger, M J W" sort="Sprenger, M J W" uniqKey="Sprenger M" first="M. J. W." last="Sprenger">M. J. W. Sprenger</name>
</author>
<author>
<name sortKey="Kletera, G E M" sort="Kletera, G E M" uniqKey="Kletera G" first="G. E. M." last="Kletera">G. E. M. Kletera</name>
</author>
<author>
<name sortKey="Beek, R Van" sort="Beek, R Van" uniqKey="Beek R" first="R. Van" last="Beek">R. Van Beek</name>
</author>
<author>
<name sortKey="Quint, W G V" sort="Quint, W G V" uniqKey="Quint W" first="W. G. V." last="Quint">W. G. V. Quint</name>
</author>
<author>
<name sortKey="Masurela, N" sort="Masurela, N" uniqKey="Masurela N" first="N." last="Masurela">N. Masurela</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:2333ADD4E632599E5B80D99083E693ED19C1EF4D</idno>
<date when="1992" year="1992">1992</date>
<idno type="doi">10.1016/0166-0934(92)90120-3</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-1190NR36-2/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000379</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000379</idno>
<idno type="wicri:Area/Istex/Curation">000379</idno>
<idno type="wicri:Area/Istex/Checkpoint">000C83</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C83</idno>
<idno type="wicri:doubleKey">0166-0934:1992:Claas E:type:specific:identification</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:1430057</idno>
<idno type="wicri:Area/PubMed/Corpus">000431</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000431</idno>
<idno type="wicri:Area/PubMed/Curation">000431</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000431</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000389</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000389</idno>
<idno type="wicri:Area/Ncbi/Merge">000085</idno>
<idno type="wicri:Area/Ncbi/Curation">000085</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000085</idno>
<idno type="wicri:doubleKey">0166-0934:1992:Claas E:type:specific:identification</idno>
<idno type="wicri:Area/Main/Merge">002028</idno>
<idno type="wicri:Area/Main/Curation">001F32</idno>
<idno type="wicri:Area/Main/Exploration">001F32</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title level="a">Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction</title>
<author>
<name sortKey="Claas, E C J" sort="Claas, E C J" uniqKey="Claas E" first="E. C. J." last="Claas">E. C. J. Claas</name>
<affiliation>
<wicri:noCountry code="subField">RotterdamNetherlands</wicri:noCountry>
</affiliation>
</author>
<author>
<name sortKey="Sprenger, M J W" sort="Sprenger, M J W" uniqKey="Sprenger M" first="M. J. W." last="Sprenger">M. J. W. Sprenger</name>
<affiliation>
<wicri:noCountry code="subField">RotterdamNetherlands</wicri:noCountry>
</affiliation>
</author>
<author>
<name sortKey="Kletera, G E M" sort="Kletera, G E M" uniqKey="Kletera G" first="G. E. M." last="Kletera">G. E. M. Kletera</name>
<affiliation>
<wicri:noCountry code="subField">RotterdamNetherlands</wicri:noCountry>
</affiliation>
</author>
<author>
<name sortKey="Beek, R Van" sort="Beek, R Van" uniqKey="Beek R" first="R. Van" last="Beek">R. Van Beek</name>
<affiliation>
<wicri:noCountry code="subField">RotterdamNetherlands</wicri:noCountry>
</affiliation>
</author>
<author>
<name sortKey="Quint, W G V" sort="Quint, W G V" uniqKey="Quint W" first="W. G. V." last="Quint">W. G. V. Quint</name>
<affiliation>
<wicri:noCountry code="subField">DelftNetherlands</wicri:noCountry>
</affiliation>
</author>
<author>
<name sortKey="Masurela, N" sort="Masurela, N" uniqKey="Masurela N" first="N." last="Masurela">N. Masurela</name>
<affiliation>
<wicri:noCountry code="subField">RotterdamNetherlands</wicri:noCountry>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series>
<title level="j">Journal of Virological Methods</title>
<title level="j" type="abbrev">VIRMET</title>
<idno type="ISSN">0166-0934</idno>
<imprint>
<publisher>ELSEVIER</publisher>
<date type="published" when="1992">1992</date>
<biblScope unit="volume">39</biblScope>
<biblScope unit="issue">1–2</biblScope>
<biblScope unit="page" from="1">1</biblScope>
<biblScope unit="page" to="13">13</biblScope>
</imprint>
<idno type="ISSN">0166-0934</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt>
<idno type="ISSN">0166-0934</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Base Sequence</term>
<term>DNA Probes</term>
<term>DNA, Viral (genetics)</term>
<term>Evaluation Studies as Topic</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Orthomyxoviridae (classification)</term>
<term>Orthomyxoviridae (genetics)</term>
<term>Orthomyxoviridae (isolation & purification)</term>
<term>Polymerase Chain Reaction (methods)</term>
<term>Polymerase Chain Reaction (statistics & numerical data)</term>
<term>RNA, Viral (genetics)</term>
<term>Sensitivity and Specificity</term>
<term>Virology (methods)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>ADN viral (génétique)</term>
<term>ARN viral (génétique)</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Orthomyxoviridae ()</term>
<term>Orthomyxoviridae (génétique)</term>
<term>Orthomyxoviridae (isolement et purification)</term>
<term>Réaction de polymérisation en chaîne ()</term>
<term>Sensibilité et spécificité</term>
<term>Sondes d'ADN</term>
<term>Séquence nucléotidique</term>
<term>Virologie ()</term>
<term>Études d'évaluation comme sujet</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>DNA, Viral</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en">
<term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>ADN viral</term>
<term>ARN viral</term>
<term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en">
<term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr">
<term>Orthomyxoviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en">
<term>Polymerase Chain Reaction</term>
<term>Virology</term>
</keywords>
<keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en">
<term>Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en">
<term>Aqueous phase</term>
<term>Assay</term>
<term>Base Sequence</term>
<term>Cdna</term>
<term>Cdna primer</term>
<term>Cdna reaction</term>
<term>Clinical sample</term>
<term>Clinical specimen</term>
<term>Clinical specimens</term>
<term>Cultured influenza strains</term>
<term>DNA Probes</term>
<term>Diagnostic purposes</term>
<term>Diagnostic value</term>
<term>Direct sequencing</term>
<term>Embryonated eggs</term>
<term>Erasmus university</term>
<term>Evaluation Studies as Topic</term>
<term>Genome</term>
<term>Genome segments coding</term>
<term>Guanidinium thiocyanate</term>
<term>Human influenza</term>
<term>Humans</term>
<term>Hybridization</term>
<term>Influenza</term>
<term>Influenza genomes</term>
<term>Influenza primers</term>
<term>Influenza virus</term>
<term>Influenza virus genomes</term>
<term>Influenza viruses</term>
<term>Mammalian virus</term>
<term>Mdck cells</term>
<term>Molecular Sequence Data</term>
<term>Monoclonal antibodies</term>
<term>Mycoplasma pneumoniae</term>
<term>Nasopharyngeal aspirates</term>
<term>National influenza center</term>
<term>Negative samples</term>
<term>Nucleic</term>
<term>Nucleic acids</term>
<term>Nucleotide sequences</term>
<term>Oligonucleotide probes</term>
<term>Plaque</term>
<term>Plaque test</term>
<term>Plaque test assay</term>
<term>Polymerase</term>
<term>Polymerase chain reaction</term>
<term>Positive patients</term>
<term>Primer</term>
<term>Primer sets</term>
<term>Rapid detection</term>
<term>Reaction mixture</term>
<term>Respiratory tract</term>
<term>Rotterdam</term>
<term>Sensitivity and Specificity</term>
<term>Serial dilutions</term>
<term>Sodium bicarbonate</term>
<term>Sodium citrate</term>
<term>Southern blot hybridization</term>
<term>Specific signals</term>
<term>University hospital</term>
<term>Virology</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Orthomyxoviridae</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Sensibilité et spécificité</term>
<term>Sondes d'ADN</term>
<term>Séquence nucléotidique</term>
<term>Virologie</term>
<term>Études d'évaluation comme sujet</term>
</keywords>
</textClass>
<langUsage>
<language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">Abstract: The aim of this study was to develop a polymerase chain reaction for specific detection of influenza A, B, and C RNA genomes. Three primer sets were selected from conserved regions of the genome coding for the non-structural proteins and were tested on 61 influenza A (22 H1N1, 9 H2N2, and 30 H3N2), 11 influenza B, and three influenza C isolates. Specific amplified products were obtained with all these strains after electrophoresis on a 2% agarose gel. The specificity of the reaction was increased by hybridization with oligonucleotide probes. When nucleic acids from a variety of micro-organisms from the respiratory tract were subjected to the PCR with these primers, no specific amplified products were generated. The sensitivity of the technique was found to be at the subpicogram level. The RNA-PCR was applied to 21 clinical specimens from patients with a culture/IF proven influenza infection. Six influenza A positive patients and 13 influenza B positive patients could be confirmed in the RNA-PCR. In two cases, influenza B positive IF specimens were found negative by the PCR. No virus could be isolated on eggs or tissue culture from these samples. RNA-PCR is a specific and sensitive technique for the detection of influenza virus genomes.</div>
</front>
</TEI>
<affiliations>
<list></list>
<tree>
<noCountry>
<name sortKey="Beek, R Van" sort="Beek, R Van" uniqKey="Beek R" first="R. Van" last="Beek">R. Van Beek</name>
<name sortKey="Claas, E C J" sort="Claas, E C J" uniqKey="Claas E" first="E. C. J." last="Claas">E. C. J. Claas</name>
<name sortKey="Kletera, G E M" sort="Kletera, G E M" uniqKey="Kletera G" first="G. E. M." last="Kletera">G. E. M. Kletera</name>
<name sortKey="Masurela, N" sort="Masurela, N" uniqKey="Masurela N" first="N." last="Masurela">N. Masurela</name>
<name sortKey="Quint, W G V" sort="Quint, W G V" uniqKey="Quint W" first="W. G. V." last="Quint">W. G. V. Quint</name>
<name sortKey="Sprenger, M J W" sort="Sprenger, M J W" uniqKey="Sprenger M" first="M. J. W." last="Sprenger">M. J. W. Sprenger</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F32 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001F32 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:2333ADD4E632599E5B80D99083E693ED19C1EF4D
   |texte=   Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction
}}

Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021